Description
A DNA sequence encoding the extracellular domain (Met1-Tyr196) of mouse CD8A (NP_001074579.1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Thr175) of mouse CD8B (NP_033988.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD8A&CD8B heterodimer was purified